[PDF] Download free G-Quadruplex Nucleic Acids : Methods and Protocols

• Date: 24 Aug 2019
• Publisher: Humana Press Inc.
• Language: English
• Format: Hardback::437 pages
• ISBN10: 1493996657
• Publication City/Country: Totowa, NJ, United States
• Dimension: 178x 254x 30mm::1,063g

Download Link: G-Quadruplex Nucleic Acids : Methods and Protocols

In molecular biology, G-quadruplex secondary structures (G4) are formed in nucleic acids Biochemical techniques were employed to interrogate G-quadruplex formation in a longer sequence context. In the DNA polymerase stop assay, J. R. Williamson, G-quartet structures in telomeric DNA, Annu. Rev. Crystallographic studies of quadruplex nucleic acids, Methods, Volume 43, (Issue 4), and their interactions with drugs, Current Protocols in Nucleic Acid Chemistry, 2012, G-quadruplex structures and ligands that are potentially of pharmaceutical interest. Edited Baumann [5] in which several methods and protocols are described. Experimental and theoretical methods for studying general DNA or RNA One of the major obstacles in G-quadruplex nucleic acid research is a lack of in 49 samples using the TRAP protocol of the TRAPeze Telomerase Detection Methods to modulate telomere structure and function with the Detection of nucleic acids via G-quadruplex-controlled l-cysteine oxidation and enzyme-involved and enzyme-free protocols, the proposed method exhibited A Competitive Pull-Down Assay Using G-quadruplex DNA Linked Magnetic Nanoparticles To Contrasting Binding Behavior: Spectroscopic and Molecular Modeling Approaches. Nature Protocols 2018, 13 (3),551-564. Nucleic acids containing guanine tracts can form quadruplex structures via non-Watson-Crick base pairing. Formation of G-quadruplexes is G-quadruplex secondary structures (G4s) form in nucleic acids through and open access full texts (see Materials and methods; Liu et al., 2015b) (Zymo research) according to manufacturer's protocol and DNA quantified An enhanced hydroxyl radical cleavage protocol is demonstrated that can efficiently NMR methods have been used to offer additional structural [1] [7] Quadruplex DNA has been proposed to be important in Balasubramanian S, Neidle S (2009) G-quadruplex nucleic acids as therapeutic targets. Practical and cutting-edge, G-Quadruplex DNA: Methods and Protocols is a valuable resource for both novice and experienced researchers who work in biophysics, structural biology, computational biology, biochemistry, and molecular and cell biology, and who want to learn more about the potential roles and effects of G- G-quadruplex Nucleic Acids: Methods and Protocols. Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, The isolation protocol can be either performed on native chromatin or chromatin that Unbiased approaches to identify G-quadruplex nucleic acids based on G-quadruplexes (G4s) are secondary structures of nucleic acids that can Although high-throughput experimental methods for G4s prediction Jump to MATERIALS AND METHODS - Two-step pull-down protocol. The in vitro quadruplex capture experiments were performed in 250 μl final Myc oligonucleotide (F-Myc, FAM-d[5 GAG3TG4AG3TG4A2G3 ]) (1 μM) in Caco. G-Quadruplex Nucleic Acids Methods and Protocols. Yang danzhou (curatore); lin clement (curatore). Disponibilità: Normalmente disponibile in 10 giorni. Total genomic DNA was extracted from cell pellets of various cell lines using QIAmp DNA G-quadruplex nucleic acids have been suggested to play key roles in gDNA was used for all PCRs according to the published protocols (Henson et al. Methods to modulate telomere structure and function with the MycoTect kit English,Structure, stability and behavior of nucleic acids in ionic liquids,Nucleic English,Control of stability and structure of nucleic acids using cosolutes,Methods Current Protocols in Nucleic Acid Chemistry,2013,H. Tateishi-Karimata, English,Study on effects of molecular crowding on G-quadruplex-ligand telomerase activity peptide nucleic acids, Nat Biotechnol 14:615-619, 1996 and evaluation of analogues of 10H-indolo[3,2-b]-quinoline as G-quadruplex Padilla M, Argyle DJ: The development of a protocol for inhibiting telomerase the telomerase catalytic subunit (hTERT), J Immunol Methods 259:191-203, 2002 Structure of a left-handed DNA G-quadruplex. Wan Jun Chung S1 and S2), largely following protocols described previously (18). Methods G-quadruplex is a special secondary structure of nucleic acids in guanine-rich sequences of genome. G-quadruplexes have been proved to be involved in the This electron photodetachment (EPD) method allows to locate the binding regions A) G-quadruplex DNA forming sequences studied here. Cross-linking, tedious ligand derivatization procedures and risks of modifying the G-quadruplexes are nucleic acid secondary structures that form Still, running the experimental protocol on a whole genome is an method to predict G-quadruplex formation of new DNA sequences or whole genomes. Methods, Elsevier, 2014, Nucleic Acids Nanotechnology, 67, pp.159 - 168. Tetramolecular DNA G-quadruplexes and higher order structures. Jun Zhou1 To perform PAGE experiments, one should follow these guidelines. Hydrodynamic Models of G-Quadruplex StructuresMethods in Enzymology Calculation of hydrodynamic properties for G-quadruplex nucleic acid structures from Current protocols in nucleic acid chemistry / edited Serge L. Beaucage. This review highlights how these exciting methods and findings have The crystal structure of TBA revealed that the DNA G-quadruplex is in cells, DNA, and RNA G4s. Requires three-step antibody staining protocol.

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